Abstract

A SIMPLE METHOD OF EXTRACTION, ISOLATION AND ANTIOXIDANT ACTIVITY OF FISETIN FROM APPLE PEELS

Syed Azhar Nizami*, Mubeen S., Zohra Jabeen, Aliya Khanum and Shifa Afreen

Abstract

Fisetin, (3,3′,4′,7-tetrahydroxyflavone), is found in various fruits and vegetables, such as strawberry, apple, persimmon, grape, onion, and cucumber etc. Several studies have demonstrated the effects of fisetin against numerous diseases such as anti carcinogenic, anti-inflammatory etc. in this study we have reported a simple method of extraction, isolation and antioxidant potential of fisetin from apple peels. For the extraction of fisetin, hydro-alcoholic mixture (methanol / water 80/20: v/v) was used, the yield was found to be 67.3%. Identification of fisetin was carried out by TLC using nhexane and ethyl acetate (40:60) as mobile phase. The Rf value of fisetin was 0.53. The isolated compound was subjected to spectral analysis by UV-Visible spectroscopy. The results of spectral analysis for fisetin revealed the peaks at 360.0 nm in DMSO. Further, Nitric oxide (NO) reducing power method was employed for invitro Antioxidant activity using DMF as a solvent at different concentrations, viz. 20, 40, 60, and 80 μg/ml and ascorbic acid was used as standard for both the methods. Results of the study suggested that fisetin from apple peels showed good to moderate Antioxidant activity.

Keywords: Polyphenols, Fisetin, Acetone extract, Antioxidant activity, TLC.