Abstract

COMPUTER-AIDED BOX–BEHNKEN OUTLOOK TOWARDS OPTIMIZATION OF EXTRACTION OF PIPERINE FROM PIPER LONGUM L. FRUITS

Isha Gupta**, Syeda Nashvia Adin**, Mohd. Aqil*, Mohd. Mujeeb*, Abdul Ahad

Abstract

Piperine, an alkaloid present in Piper longum fruits possesses multifaceted pharmacological properties. The study compares the efficacy of different modern and conventional methods for piperine extraction and demonstrates the process parameter at which high yield of piperine can be extracted from Piper longum fruits in "one run". Box-Behnken Design, was availed for optimizing the process parameters for the extraction of piperine from Piper longum fruits. Extraction conditions (extraction time, solvent-to-drug ratio and extraction temperature) were optimized by Response-Surface Methodology (RSM), specifically BBD. Quantification analysis of piperine in different extracts was done using HPLC. From experimental results, the reflux technique stood out to be the best amongst all the other thermal and non-thermal modes of extraction used, and ethanol was proved to be the most efficient extracting solvent. Through the use of BBD, the optimal conditions for piperine extraction were established as extraction temperature- 62.714 °C, solvent-to-drug ratio- 23.939 mL/g, and extraction time- 65.514 minutes. Under such conditions, piperine was yielded as 31.57 mg/g, which was nearly close to the predicted value of 32.315 mg/g.

Keywords: Piperine, Piper longum, Extraction, Optimization, Response surface methodology.