Abstract

EVALUATION OF NEUROPROTECTIVE EFFECT OF NEOHESPERIDIN DIHYDROCHALCONE FOLLOWING EXPERIMENTAL PARTIAL SCIATIC NERVE LIGATION IN WISTAR RATS

Zainab Ruksar* and Dr. Lalitha Devi Atluri

Abstract

The bitter orange component Neohesperidin dihydrochalcone (NHDC), which is semi-natural, is a potent sweetener. NHDC shows diverse bioactivities including antioxidant, free radical scavenging activity, ulcer protective activity, anti- adipogenic activity and hepatoprotective activity but it was not evaluated in the chronic neuropathic pain, partial sciatic nerve ligation (pSNL) in rat model by using pregabalin (PREG) as standard. The main objective of the present study is to evaluate the Neuroprotective effect of ―Neohesperidin dihydrochalcone‖ followed experimental partial sciatic nerve ligation in Wistar rats. This study was intended for 20 days, using male Wistar rats. Animals were randomized into six groups (n=6). Normal control, sham control, disease control, treatment group: low dose - NHDC (40 mg/kg, P.O.), high dose –NHDC (80 mg/kg, P.O.), standard - pregabalin (10 mg/kg, P.O.) along with PSNL. During the treatment period, behavioral parameters were measured on 7th, 14th and 22nd day and the animals were subjected to estimation of biochemical parameters (MDA, SOD and Total Protein content) on 7th day and 14th day using plasma and on 22nd day using sciatic nerve tissue homogenate. Administration of NHDC resulted in the dose dependent attenuation in pSNL-induced behavioral and biochemical parameters. Consequently, it can be concluded that neuroprotective effect of NHDC in rats after pSNL may be attributed to various oxidative markers as well as the pro-inflammatory mediators secreted at the injury site. NHDC appears to be a promising

Keywords: Partial sciatic nerve ligation (pSNL), Neohesperidin dihydrochalcone (NHDC), pregabalin, neuroprotective activity hyperalgesia, pro-inflammatory mediators.