Abstract

EVALUATION OF EFFICACY OF SELECTED PLANT EXTRACTS ON SEED GERMINATION OF GREEN GRAM

Marhoob Banu, Prathibha K. Y., Nalini T. J., Geetanjali R., Suresh Kumar C., Khamer Ferzana Banu and Keshamma E.*

Abstract

The current study was conducted with the main purpose to assess four plant extracts viz. Azadirachta indica (Neem), Costus pictus (Insulin plant), Ocimum tenuiflorum (Tulsi) and Trigonella foenum (Fenugreek) at concentration 20%, 40%, 60%, 80%, 100% and 0% (untreated seeds were used as control) treated for seed germination on green gram seeds. Plant extract was prepared by grinding 200 grams of fresh and healthy leaves in 100 ml of distilled water and these leaf extracts were used to evaluate germination percentage of mung bean seeds. Green gram seeds were soaked for 24 hours in a distilled water. After 24 hours the seeds were treated in the leaf extracts of different concentrations for 2 hours. After 2 hours of seed treatment the seeds were transferred to the petriplates in the following manner- 2 in the centre, 8 in the 1st circle and 15 in the 2nd circle (Standard blotting method). The petriplates were kept at normal day light and at room temperature. Drops of water were added to the plates twice a day and the seed germination was observed for 7 days. Experiments were conducted in the closed petriplates and in triplicates. Germination of seeds was observed on daily basis for 7 days. The mean root length, shoot length, leaf length, wet weight and dry weight were recorded after 7 days. The seeds with full growth of radical and plumule were considered normal. The germination percentages were recorded. Results revealed that the Trigonella foenum extract at 100% concentration showed increase in the mean root, shoot and leaf length. The shoot length and root length of the seedlings of Green gram was inhibited by the plants extracts of Ocimum tenuiflorum. Azadirachta indica and Costus pictus.

Keywords: Seed germination, Plant extracts, Green gram, Seedlings, Standard blotting method.