Abstract

SOLVENT STANDARDIZATION OF MUCUNA PRURIENS LEAF EXTRACTS FOR CONTENT OF TANNINS AND EVALUATION OF POTENCY AGAINST K. PNEUMONIAE

Gauri Risbud*, Pralhad Rege, Sayali Kadge, Sachin Palekar and Deepika Jain

Abstract

Mucuna pruriens commonly known as velvet beans is a tropical legume belonging to the fabaceae family which is native to tropical. Fabaceae or Leguminosae family is widely known for its nutritional factors and different constituents supporting the therapeutic importance. Extraction of phytoconstituents and standardization of solvents for getting maximum yield is of paramount importance in the world of Natural Therapeutics. We are trying to summarize the overall impact of different solvents on tannin content and the its antimicrobial potential along with Minimum Inhibitory Concentration (MIC) against Klebsiella pneumoniae. Tannins are a group of polyphenols that astringent in nature. Tannins possess anti-inflammatory, anti-oxidant, anti-microbial and other activities. In the current paper, we are focusing upon the content of tannins colorimetrically. The Folin-Denis method is used for quantification of tannins. Phenolic hydroxyl groups present in the structure of tannins reduce phosphotungstomolybdic acid to phosphotungstomolybdate in the presence of alkaline reagents like sodium carbonate solution. Tannins act as antimicrobial agents as they inhibit extracellular microbial enzymes which are responsible for microbial metabolism. The organism is specifically chosen as the plant family used is suspected to contain some potential analytes against it. Hence present work also highlights the antimicrobial potential by disc diffusion method and further evaluation of MIC of leaf extracts prepared in different solvents.

Keywords: Mucuna pruriens, tannins, extraction, Disc diffusion method, MIC.