Abstract

REVIEW ON ISOLATION AND PURIFICATION OF PROTEIN

Akbar A. Shaikh*, Priyanka Deshmukh, Archana Chikne, Kuldeep Kanadje, Ashwini Chandile

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Abstract

.Proteins are large, complex molecules that play many critical roles in living organisms, including plants. Even though the content of protein is considered to be low in plants, their contribution in many stages of growth and development, which take place throughout a plant‘s life, is tremendously significant. Plants have to take up low-molecular-weight nitrogen compounds such as ammonium, nitrate, and amino acids as precursors in the synthesis of protein. When the process of synthesis is completed, they are assembled into proper folding and deposited at certain plant organs. The occurrence of protein in legumes, such as soybeans, and some other vegetables demonstrates higher protein content as compared to fruits. Their presence may have positive and negative effects on human health. Several methods have been developed to measure the protein content in food. At the molecular level, proteomics analysis may provide a platform to allow their identification and give accurate profiles of proteins of interest.

Keywords: Proteins, Amino acid, Spike Proteins, Chromatography, Precipitation.