Abstract

REVIEW ON ANIMAL CELL CULTURE TECHNIQUE: THEORY

Shubhada S. Pawar, Sanjay K. Bais and Prachi Pandit Gaikwad*

Abstract

A thorough analysis of animal cell culture techniques, tools, and media formulation, sterilisation, and culture vessels & vessels, Organotypic culture and specialised cell culture techniques; various tissue culture techniques; primary culture, secondary culture, contamination, cryopreservation & cytotoxicity Cell culture techniques used in drug development and evaluation include trypsinization, cell separation, suspension culture, organ culture, and cryopreservation. Modern life sciences require the use of animal cell culture technology, which serves as a foundation for research into regulation, proliferation, and differentiation as well as genetic manipulation. The crucial background knowledge needed to establish animal cell cultures is included in this overview. Cancer research, vaccine production, recombinant protein synthesis, medication development and selection, gene therapy, stem cell biology, monoclonal antibody production, in vitro fertilisation technology, cryopreservation, and in vitro hormone production are some of the main applications. The overview of the idea of animal cell culture and its fundamental details are covered in this paper.

Keywords: Equipment, aseptic techniques, Primary culture, secondary culture, contamination, cytotoxicity, Culture vessels and media development, Trypsinization, Cryopreservation, Applications.