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Abstract

WATER QUALITY & METHOD VALIDATION FOR DETECTION OF CIPROFLOXACIN RESIDUES IN HOSPITAL'S DRINKING WATER IN KHARTOUM STATE, SUDAN

Hamed H.A.* and Prof. Abbo M.A.

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Abstract

This study describes a simple salting-out liquid–liquid extraction technique for detection of ciprofloxacin (CIP) residue in 28drinking water samples from Suba & Ibrahim Malek hospitals. The highperformance liquid chromatographic ultra violet visible detection (HPLC-UV) containing C18 X-bridge analytical column was used for the determination of this antibiotic in water samples. The range of concentration of CIP in samples was between 0-928μg/ml. The developed method has linear range of 50-500μg/ml with the linearity coefficient of 0.9989. The limit of detection and limit of quantification of the method for ciprofloxacin was 61.2 and 185.5μg/ml respectively. The recoveries for the studied antibiotic ranged from 22.9-151.6%. The results also showed that the 6 physicochemical parameters of (pH, EC, TDS, turbidity, water-hardness, and some acidic radicals) were not adhering to, or extremely higher than the stipulated World Health Organization (WHO) standards.

Keywords: Ciprofloxacin, HPLC-UV, Limit of detection, Limit of quantification, Recovery.


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