Abstract

OSTEOGENIC POTENTIAL OF FICUS RELIGIOSA BARK IN FEMALE OVARIECTOMIZED SPRAGUE DAWLEY RATS

*Farhad Siddiqui, Shafkeen Siddiqui, Zeashan Hussain

Abstract

Aim: Investigation of osteogenic potential of Ficus religiosa bark in female ovariectomized Sprague Dawley rats. Material and method: The bark of Ficus religiosa was tested for anti-osteoporotic activity in a female ovariectomized Sprague Dawley rat model. The rats were divided into five groups (n=6) such as Normal Control (NC), Disease Control (DC), Treatment-1 (low dose), Treatment-2 (high dose), and Standard (Std.) group. NC and DC were given 1% CMC, Treatment-1 was given 25mg/kg/day dose of 70% ethanolic extract of Ficus religiosa bark Treatment-2 was given 50mg/kg/day dose of 70% ethanolic extract of Ficus religiosa bark and Standard was given Alendronate (0.2mg/kg/day) orally for 28 days. Result: On the basis of change in serum biomarkers, bone turnover and bone mineral density (BMD), 50mg/kg/day dose of 70% ethanolic extract of Ficus religiosa bark was better in preventing ovariectomy induced osteoporosis and showed almost similar therapeutic effect as 0.2mg/kg/day dose of Alendronate. Conclusion: The current study proved that 50mg/kg/day dose of Ficus religiosa bark alleviated the bone porosity and complexity of the bones due to its osteoblastic effect and antioxidant effect.

Keywords: OVX, osteoporosis, bone turnover, CMC, BMD.