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Abstract

HPTLC FINGERPRINTING AND PHYTOCHEMICAL ANALYSIS OF TEJOVATI ZANTHOXYLUM ARMATUM DC.

Deeksha*, Kiran Vashisht and D. C. Singh

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Abstract

Introduction: Oral health is of prime importance to all individuals and oral hygiene habits are instilled in childhood itself irrespective of the nationality or geographic location of an individual. Oral diseases are perhaps, the most widespread of all diseases prevalent in the world. No population is free from caries and periodontal diseases – the most common of all such diseases and affecting almost 80% of the population. Poor oral hygiene or tooth disorders are very common and severely agonizing ailment leads to teeth loss or damaging of teeth, so it is the need of the hour to search for a potent drug which can fight this condition. Tejovati is first described in ayurvedic text Charak Samhita as in Shirovirechan dravyas. Later in some medicinal books Tejovati is mentioned as potent mediciene for Dantvikar, agnimandaya, udarshoola etc. Zanthoxylum armatum DC. of Rutaceae family is an alpine medicinal plant that is found in temperate Himalaya between 2000-3000 m The fruit of the plant is of good medicinal value and has been used as folk remedy for treating ailments like diabetes mellitus, gastric problems, peptic ulcers, diarrhoea, cancer, toothache, pyorrhoea, cough, cold and also has anthelmintic property. Methods: An attempt has been made to study the macroscopic and microscopic character, physicochemical analysis, phtochemical analysis, and chromatographic studies of its root following standard procedures. Two different extracts of Tejovati fruit were used to access the physicochemical and HPTLC analysis. Research work was carried out as per standard operating procedures and specified protocols. Result: Results showed that in physicochemical evaluation aqueous extract 2.9%, hydro-ethanol extract 3.6%, total ash 8.5%, water soluble ash 3.5%, acid and insoluble ash 2.0%. HPTLC results for the quantification of linalool in EPF was analyzed for the first time by scanning at wavelength (λmax 366 nm) and the quantity of linalool present in Tejovati was estimated to be 1.48% w/w. In phytochemical analysis presence of carbohydrate, protein, glycosides, amino acid, protein in extract of Tejovati. Conclusion: Tejovati is a highly potent medicine for oral diseases due to presence of monoterpenes like linalool and limonene.

Keywords: Tejovati, Dantvikar, pharmacognostical, TLC, HPTLC.


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