Abstract

SPECTROPHOTOMETRIC DETERMINATION OF DRUGS BY USING CHLORAMINE-T AND METHYL ORANGE

M. Sasikala, K. Sayanna and G.Venkateshwarlu*

Abstract

Simple and sensitive validated spectrophotometric methods has been developed for the assay of five drugs viz., Gemifloxacin (GEM), Ondensetron (OND), Alfuzocin (ALF), Dulaxetine (DUL) and Torsemide (TOR) in pure and pharmaceutical formulations. The proposed method was based on the oxidation of drug by Chloramine-T (excess) and subsequent determination of unreacted Chloramine-T using methyl orange as analytical tool which absorbs at 510 nm. Beer’s law is obeyed in the concentration of 2-14 , 4-28 , 3-24 , 3-21 and 1.5- 8.5 μg mL-1 for GEM, OND, ALF, DUL and TOR respectively. Different variables affecting the reaction were studied and optimized. The proposed methods were applied successfully to the determination of the examined drugs in pure and pharmaceutical dosage forms with good accuracy and precision.

Keywords: spectrophotometry, Chloramine-T, methyl orange and formulations.