Abstract

DETERMINATION OF THE TOTAL AMOUNT OF FLAVONOIDS IN THE FRUITS OF CRATAEGUS OXYACANTHA L (FAMILY: ROSACEAE)

N. Gelovani*, I. Gvelesian, L. Lomaia, G. Pataridze, I. Goderdzishvili and Kh. Tsikarishvili

Abstract

Biologically active substances belong to the products of secondary metabolism, which are called secondary metabolites or secondary products of biosynthesis. Currently, more than 100,000 secondary metabolites produced by plants are known. Many of them are practically, economically important products and are used in the pharmaceutical, cosmetic and food industries. Objectives: Determination of the total amount of flavonoids in the fruits of Crataegus oxyacantha L (family: Rosaceae) Depending on the chemical structure of flavonoids, they are divided into the following subclasses: Anthocyanins, Chalcones, Flavanones, Flavones, Flavonols, Flavan-3-ol, Isoflavones. Methods: For flavonoids, as for other substances, there is no isolation method that is universal for all plants. We have searched the literature in order to select the methods of isolation, concentration and determination of flavonoids and their complexes of Crataegus oxyacantha common in Georgia. The beneficial properties of Crataegus oxyacantha L are due to the biologically active substances contained in it. Studies have shown that different types of Crataegus oxyacantha L are similar in chemical composition and have very characteristic physiological effects on the living organism. Results: We conducted a complete chemical analysis of the plant Crataegus oxyacantha L. The chemical composition of our samples per 100 grams of fruit is as follows: proteins 1.12g; carbohydrates 14.2 g; organic acids .33 g; Dietary fibers 6.5 g. The results of the analysis of the ash obtained from fruits Crataegus oxyacantha L(calculated per 100 grams) are as follows: water 72 g, ash 2.73 g; Coexistence of micro and macro elements in ash is confirmed. We determined the total content of phenolic compounds in the fruits of Crataegus oxyacantha L by thin-layer chromatography and quantified the total flavonoids - by the spectral method (with AlCl3 reagent, converted to hesperidin). Conclusion and Recommendation: Chromatogram analysis of rutin standard solution revealed that all selected fractions of our three samples contained rutin.

Keywords: Crataegus oxyacantha L plant, flavonoids, determination of the amount of total flavonoids; Chromatographic analysis, rutin, hyperoside, hyperin, tannins, sorbitol, choline and fatty oil.