Abstract

STATISTIC ANALYSIS & METHOD VALIDATION FOR DETECTION OF CIPROFLOXACIN RESIDUES IN HOSPITAL'S DRINKING WATER IN KHARTOUM STATE, SUDAN

Hamed H. A.* and Abbo M. A.

Abstract

This study describes a simple salting-out liquid–liquid extraction technique for simultaneously detection of ciprofloxacin residue in 41drinking water samples from four hospitals (Alacademy & Eltamouz, Ibrahim Malek & Suba). The high-performance liquid chromatographic ultra violet visible detection (HPLC-UV) containing C18 X-bridge analytical column was used for the determination of this antibiotic in water samples. The range of concentration of CIP in samples was between 0-928μg/ml. The developed method has linear range of 50-500μg/ml with the linearity coefficient of 0.9989. The limit of detection and limit of quantification of the method for ciprofloxacin was 61.2 and 185.5μg/ml respectively. The recoveries for the studied antibiotic ranged from 22.9-151.6% with relative standard deviations between 0.62 and 1.19%. The observed compatibility and deviations were determined by comparing the results statistically using various statistical programs SPSS PASW-23, and significant differences accepted at p≤ 0.05.

Keywords: Ciprofloxacin; HPLC-UV; Limit of detection; Limit of quantification.