Abstract

INVITRO ANTI-BACTERIAL, ANTI-TUBERCULOSIS AND ANTIMALARIAL ACTIVITY OF GUNJA SEEDS

S. Dharmaraj Santhosam*, P. Selvam and Abhinandan Danodia

Abstract

Abrus precatorius is also called as Gunja. The research work aims to determine the In-Vitro Antibacterial, Antimalarial, and Antituberculosis activity of the methanol crude extract of Gunja seeds CMME and its isolated compounds CMME I, CMME II, and CMME III. The methanol crude extract CMME was prepared by the maceration method for 7 days. The compounds from the crude extract were isolated by column chromatography using silica gel 60-120 mesh as the adsorbent and methanol as the mobile phase. The isolated compounds are further purified by Thin Layer Chromatography. The zone of inhibition was measured by the plate well diffusion method against the multi drug resistant bacteria. The isolated compound CMME I consists of more zone of inhibition against Klebsiella Pneumonia compared to CMME, CMME II, and CMME III. The zone of inhibition of the CMME I was found to be 28 mm. The isolated compound CMME III consists of more zone of inhibition against E.Coli compared to CMME, CMME I, and CMME II. The zone of Inhibition of CMME III was found to be 26 mm. The isolated compound CMME I consists of more zone of inhibition against Staphylococcus Aureus compared to CMME, CMME II, and CMME III. The zone of inhibition of CMME I was found to be 15 mm. The minimum inhibitory concentration of the standard Isoniazid, Rifampicin, and Ethambutol was found to be 0.05μg/ml, 0.1μg/ml, and 1.56mcg/ml respectively. The minimum inhibitory concentration of the CMME, CMME I, CMME II, and CMME III was found to be > 25μg/ml. The CMME and its isolated compounds do not have any significant activity against Mycobacterium tuberculosis H37Rv. The crude extract CMME and the isolated compounds CMME I, CMME II, and CMME III are carried out for the Semi-quantitative Test for Screening of Anti-malarial Activity. But they did not show more activity as Antimalarial as compared to the standard drug Chloroquine phosphate and they contain low Anti-malarial Activity.

Keywords: Multidrug-resistant bacteria, Plate well diffusion method, Mycobacterium tuberculosis, Malaria.