Abstract

PHYSICOCHEMICAL AND IMMUNOPROTEOMIC ANALYSIS TO DESIGN SYNTHETIC PEPTIDE VACCINE FROM NAJA KAOUTHIA NEUROTOXIN

Gomase V. S.*, Kemkar K. R., Baviskar B. A., Mundhe V. S., Sakhare A. D., Kolsure A. K., Bhimanwar A. A., Dhamane S. P. and Potnis V. V.

Abstract

N. kaouthia bite can cause body paralysis or even death due to respiratory failure. Weak neurotoxin from N. kaouthia is used to find out potential binding peptides. In this assay, we used PSSM and SVM algorithms to detemine antigenic epitope and predicted the binding affinity of toxin protein having 86 residues, which shows 78 nonamers. In addition we have predicted the several high affinity TAP-binders on the basis of amino acid sequence and properties. Binding ability prediction of antigen peptides to major histocompatibility complex (MHC) class I & II molecules is important in vaccine development from N. kaouthia weak neurotoxin.

Keywords: Neurotoxin, Epitope, Nonamers, PSSM, SVM, MHC, TAP.