Abstract

EVALUATION OF DELONIX REGIA (LEAVES) WAS USED TO INVESTIGATE FOR THEIR PROTECTIVE ABILITY OF THE KIDNEY AGAINST GENTAMYCIN-INDUCED NEPHROTOXICITY

Satendra Dhakad*, Dr. Ravi Prakash Yadav, Dr. Brajesh Ku. Arjariya, Rahim Khan, Mohd. Jalaluddin and Laxminarayan Dhakad

Abstract

The present study aims to examine the potential beneficial effects of Delonix regia (leaves) against gentamicin-induced renal injury in vitro and in vivo, as well as to investigate the main underlying pathogenic mechanisms. Gentamycin is a widely used aminoglycoside antibiotic that has proven efficiency against life-threatening Gram-negative bacterial infections. Collected material was washed thoroughly in running tap water, rinsed in distilled water and shade dried in open air and ground to powder. The air-dried and powdered defatted marc of the drugs was subjected to extraction with hydroalcoholic solvent (ethanol: water: 80: 20). Tested for the presence of alkaloids, glycosides, flavonoids, and carbohydrates etc. In conclusion Preliminary phtochemical investigation on 80:20 hydroalcoholic extract of Delonix regia leaves showed the presence of bioactive compounds such as Glycosides, flavonoids, diterpenes, phenolics, carbohydrates, saponins compounds and alkaloids, proteins and amino acids are absent. A group of natural polyphenols called “flavanoids” are of most popular interest because reserchers have found them to be of health contributing potenial. The total phenol content was 1.690 (mg GAE/100mg of leaves extract while the flavonoid content was 1.305 (mg QE/100mg) of hydroalacolic extract. Therefore, Delonix regia leaves represented a novel therapeutic tool in the prevention and treatment of gentamicin-induced nephrotoxicity in the clinical setting.

Keywords: Gentamycin, nephrotoxicity, Delonix regia, kidney.