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Abstract

IN-VITRO ANTIOXIDANT ACTIVITY OF HERBAL DRUGS

Kotresh Yaligar* and Dr. Akhlesh K. Singhai

Abstract

The aim & objective of the research is to evaluate the potential antioxidant activity of herbal drugs such as Gymnema Sylvestre (Leaves), Trigonella Foenum-Gr (Leaves), Tinospora Cardifolia (Stems), Azadirachata Indica (Leaves), Cinnamomum Zeylanicum (Stem Barks), Syzygium Jambolana (Fruits) & Nardostachys Jatamansi (Roots) which are well-known medicinal plants available throughout India and they are commonly used for the treatment of various diseases. All the herbal drugs were collected from herbal shop (Grovel Drugs & Chemicals Pvt Ltd. Hyderabad). The quality of the finished product was evaluated as per the World Health Organization's guidelines for the quality control of herbal materials. The quality testing parameters of the herbal drugs were within the limits. The herbal drugs were subjected for extraction with ethanol (90%) by cold maceration & soxhelation method to get ethanolic extracts. The ethanolic extracts of herbal drugs were evaluated for its antioxidant activity. Screening of preliminary phytochemical investigation of herbal extracts shows the presence of large amounts of phenolic, tannins & flavonoid compounds which exhibits the highest antioxidant and free radical scavenging and also inhibited lipid peroxidation. A positive correlation was observed between phenolic content and free radical (DPPH and OH) scavenging efficiencies and lipid peroxidation inhibition activity. All the herbal extracts shows promising effect of antioxidant activity and it may be used as an alternative remedy for the treatment of diabetes mellitus.

Keywords: Herbal drugs, Antioxidant, Flavonoids, Ethanol, etc.


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