Abstract

EVALUATION OF PHYTOCHEMICAL AND ANTI- MICROBIAL SCREENING OF METHANOLIC EXTRACT OF LIMONIA ACIDISSIMA FRUIT

Dishi Sahu*, Barkha Chaturvedi, Manisha Verma, Nirbhik Karan and Prashanasa Tripathi

Abstract

The objective of the present study was to investigate the presence of phytochemicals from the methanolic extract of Limonia acidissima L. Fruit and their anti-microbial action against various Gram-positive and Gram-negative bacteria. Herbal Medicines are the major remedy for traditional system of medicine have been used in medical Practices since ancient times. . Limonia acidissima L. belongs to a monotypic genus Limonia and is classified under the family Rutaceae. It is an exotic deciduous tree found in India, Pakistan, Sri Lanka and Southeast Asia. The Potential antimicrobial activity was examined by minimum concentration and zone of inhibition Analysis. The antimicrobial test was carried out using disk diffusion methods. Phytochemical screening results of ethanol fraction Limonia acidissima can be detected in the content of alkaloids, terpenoids, anthraquinones, and saponins. Methanol extract showed good antibacterial activity with the high inhibition zones.

Keywords: Traditional, Limonia acidissima, phytochemicals, antimicrobial, disk diffusion.