FLOW CYTOMETRIC ANALYSIS OF IMMUNOADJUVANT ACTIVITY OF EMBLICA OFFICINALIS ON HUMAN WHOLE BLOOD
Amit Gupta and Sushama R Chaphalkar*
Abstract
In this study, alkaloid fraction from Emblica officinalis was evaluated
for its potential ability as an adjuvant effect on the immune responses
to hepatitis and diphtheria-pertussis-tetanus (DPT) antigen on human
whole blood using flow cytometry. Cells were treated with variable
doses of alkaloid fraction (0.625 – 2.5 mg) in presence or absence of
hepatitis and DPT vaccine antigen. Hepatitis and DTP vaccine
containing alum used as standard for these studies. The results showed
that the DPT mediated blood counts were significantly enhanced by
alkaloid fraction at lower doses (0.625 mg) compared with DPT
control group. Moreover, alkaloid containing hepatitis antigen showed
no adjuvant effect on human whole blood.
Keywords: Emblica officinalis, hepatitis,
[Full Text Article]