Abstract

DE-TANNIFICATION OF PUNICA GRANATUM L. JUICE USING TANNASE PRODUCED FROM A LOCALLY ISOLATED MUCOR ELLIPSOIDEUS126271

Sunanda Joshi and Arindam Kuila*

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Abstract

Bioprocesses for synthesizing tannase enzyme help add value to agroindustrial waste. This study aimed to investigate a new filamentous fungus strain isolated from the intestines of Odontotermes obesus (white Indian termite) for tannin acyl hydrolase (Tannase) production and characterize it using 16S rRNA gene sequencing technology. The synthesis of tannase was boosted in a submerged fermentation medium combining household and industrial agro-wastes, i.e., Ananas comosus and Citrus limetta peel substrate, in a 1:1 ratio. After four days of incubation at 35°C and a 6 % substrate concentration, the optimal tannase yield (0.690 IU/mL) was obtained. A sequential optimization strategy based on a statistical research design was utilized to optimize the result of tannase enzyme by Mucor ellipsoideus (M.ellipsoideus) strain CBS 126271 by submerged fermentation. The enzymatic saccharification was modified using central composite method (CCD)-based response surface methodology (RSM), and statistical analysis of variance (ANOVA) revealed a satisfactory correlation between the experimental parameters chosen. It was discovered that purification with 60% ammonium sulphate treatment was sufficient for maximum tannase activity. For clarity and quality enhancement, pomegranate (Punica granatum L.) juice was treated with crude extracts of tannase enzyme generated in the lab. When different amounts of tannase were treated with pomegranate juice at 35°C, a 25% decrease in tannin was detected without losing biological attributes such as pH, sugar content, or protein content.

Keywords: Tannase, Tannin, Termite, Submerged fermentation, Juice Clarification, M. ellipsoideus