Abstract

“PHYTOCHEMICAL INVESTIGATION, HPTLC METHOD DEVELOPMENT AND PHARMACOLOGICAL SCREENING OF EXTRACT LEAVES OF CASSIA SENNA SOPHERA LINN”

Prakash G. Korwar*, Lingaraj S. Danki and Prabhudev S. M.

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Abstract

HPTLC is a perfect analytical tool which has been used for qualitative and quantitative estimation of active markers. HPTLC fingerprint is the applicable and critical parameter associated with the standardization of crude drugs. Densitometric HPTLC has been widely used for the phytochemical evaluation of the herbal drugs, due to its simplicity and minimum sample clean up requirement. Hence, a densitometric HPTLC method has been developed in the present work for quantitation of gallic acid from hydroalcoholic extract of dried leaves of Cassia sennasopheralinn. The phytochemical analysis of ethanolic, aqueous and hydro alcoholic extract suggests the presence of flavonoids the prominent compound was developed by HPTLC method using nonpolar and polar solvent systems in the form of different density gradients. Finally the Quercetin is subjected to various spectral analyses such as UV, IR and H1 NMR. The spectral data was characterized for the structural elucidation of the molecule. Ethanolic, aqueous and hydro alcoholic extract were used for the evaluation for the anti-bacterial activity. In the present study Gentamycin used as standard and gram positive & gram negative bacteria used.

Keywords: HPTLC, Phytochemical Evaluation, Cassia SennaSophera Linn. Quercetin, Anti-Bacterial Activity.