Abstract

PHARMACOGNOSTIC STUDY AND PRELIMINARY PHYTOCHEMICAL ANALYSIS OF KALIHARI (Gloriosa superba Linn.) TUBEROUS ROOT

*Sanjay Pratap Singh, Rajesh Garg and Manoj Tripathi

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Abstract

Kalihari (Gloriosa superba L..) Family Liliaceae is known as economical and medicinal values. It is a semi-woody herbaceous climber. It is grown as ornamental plants and found throughout India up to an altitude of 6000 fits. It is used in traditional medicinal and ayurvedic system of medicines. All parts of the plant are used for the treatment of various human as well as disorders. The present communication provides a detailed account of the pharmacognostic study carried out of Kalihari root. The study includes macroscopy, microscopy and powder microscopic studies, preliminary phytochemical investigation, physicochemical tests, heavy metal tests, screening of microbiological parameters and development of HPTLC (High Performance Thin Layer Chromatography) fingerprints profile. Physicochemical parameters were performed and found LOD was found 5.84% w/w, total ash value 10.16% w/w, acid insoluble ash value 0.21% w/w, alcohol soluble extractive value 13.98% w/w and water soluble extractive value 25.77%w/w. HPTLC (High Performance Thin Layer Chromatography) fingerprints profile of methanolic extract was done by using mobile phase toluene: ethyl acetate (7:3). TLC plate was derivatized by using 5% Methanolic-sulphuric acid derivatizing reagent. Major spots Rf values and colour were recorded at 366nm, after derivatization 366nm and UV light. Quantitative microbiological tests were performed and specific pathogens found absent such as Staphylococcus aureus/gm, Salmonella sp./gm, Pseudomonas aeruginosa/gm, Escherichia coli, where total microbial count (TBC), and Yeast & Mould found under WHO limits. Heavy metals such as Pb, Cd, As, & Hg were tested and found under WHO limits Established parameters can be used as standards for quality control and identification of the plant in herbal compound formulations and also preparation of a monograph of the plant.

Keywords: Kalihari, Pharmacognostic, Physicochemical, Phyto-chemical analysis, HPTLC.