RP-HPLC METHOD DEVELOPMENT FOR THE QUANTITATIVE DETERMINATION OF DEXAMETHASONE IN HERBAL FORMULATION
Venkata Sairam K*, J C Thejaswini, M V Prudhvi Raju, R S Chandan,
B M Gurupadayya, K Mruthunjaya
Abstract
A simple, sensitive method for the determination of dexamethasone
used as an adulterant in herbal formulation was developed using
reverse phase high performance liquid chromatographic method (RPHPLC).
The analysis was performed on cyano (250 × 4.6mm, 5μm)
column using 0.1% ortho phosphoric acid and acetonitrile (80: 20 v/v)
as mobile phase at flow rate 1.2 mL/min. The eluents were monitored
with UV detector at 242 nm. In the developed method dexamethasone
elutes at a retention time of 8.8 min. The proposed method is having
linearity in the concentration range from 5 to 60 μg/mL of
dexamethasone. The present method was validated with respect to
system suitability, linearity, precision, limit of detection (LOD) and
limit of quantification (LOQ), accuracy (recovery) and robustness. The
proposed method can be readily utilized for the detection of
dexamethasone as an adulterant in herbal formulation.
Keywords: Dexamethasone, Herbal formulation, RP-HPLC.
[Full Text Article]