Abstract

RP-HPLC METHOD DEVELOPMENT FOR THE QUANTITATIVE DETERMINATION OF DEXAMETHASONE IN HERBAL FORMULATION

Venkata Sairam K*, J C Thejaswini, M V Prudhvi Raju, R S Chandan,
B M Gurupadayya, K Mruthunjaya

Abstract

A simple, sensitive method for the determination of dexamethasone used as an adulterant in herbal formulation was developed using reverse phase high performance liquid chromatographic method (RPHPLC). The analysis was performed on cyano (250 × 4.6mm, 5μm) column using 0.1% ortho phosphoric acid and acetonitrile (80: 20 v/v) as mobile phase at flow rate 1.2 mL/min. The eluents were monitored with UV detector at 242 nm. In the developed method dexamethasone elutes at a retention time of 8.8 min. The proposed method is having linearity in the concentration range from 5 to 60 μg/mL of dexamethasone. The present method was validated with respect to system suitability, linearity, precision, limit of detection (LOD) and limit of quantification (LOQ), accuracy (recovery) and robustness. The proposed method can be readily utilized for the detection of dexamethasone as an adulterant in herbal formulation.

Keywords: Dexamethasone, Herbal formulation, RP-HPLC.