Abstract

OPTIMIZED BIOREACTOR TECHNIQUES IN THE PRODUCTION AND PURIFICATION OF TETRACYCLINE FROM “STREPTOMYCES AUREOFACIENS”

Dr. Prasad.M.P*

Abstract

The Tetracycline’s are a family of antibiotics that inhibit protein synthesis by preventing the attachment of aminoacyl-tRNA to the ribosomal acceptor (A) site. The favourable antimicrobial properties of tetracycline and the absence of major adverse side effects have led to their extensive use in the therapy of human and animal infections. The spectrum of activity of tetracyclines encompasses various protozoan parasites such as P. falciparum, Entamoeba histolytica, Giardia lamblia, Leishmania major, Trichomonas vaginalis, and Toxoplasma gondii. In the present study Streptomyces aureofaciens was isolated on Waksman agar media and Actinomycetes isolation agar from different sources of soil and screened for the production of tetracycline. The isolate and media for production of tetracycline was optimization for different physical and chemical parameters like pH, temperature, carbon source and nitrogen source for maximum production of tetracycline and the mass production was carried out with the parameters that are conformed in the optimization results. The mass production media were prepared with the pH 9, carbon source as dextrose, nitrogen sources as yeast extract and temperature were carried out at 350C with rpm 200 and aeration limit was 131units/min. Olive oil was used as the antifoaming agent. The mass produced broth was collected and the tetracycline was purified and checked for antimicrobial activity against E.coli and the zone of inhibition was found to be 30mm in diameter. The zone of inhibition was greater in the optimised media when compared to the normal production media. This indicates the capability of this strain in mass production and commercial use of this antibiotic against drug resistant pathogens.

Keywords: Tetracycline, Optimisation, Streptomyces aureofaciens, E.coli.