IMPACT OF THE TRANSCRIPTION FACTOR 7-LIKE2 (TCF7L2) GENE POLYMORPHISMS ON THE RISK OF POLYCYSTIC OVARY SYNDROME IN IRAQI PATIENTS
Mohammed T. Al-Tamimi, Ismail.A.Abdul-Hassan*, Saad.S.Al-dujaily and Bushra J.M.Al-musawi
Abstract
The relationship of TCF7L2 gene polymorphisms (rs7903146)
with the incidence of PCOS and diabetic PCOS in Iraqi patients
(women) was evaluated in this study. Sixty PCOS and diabetic PCOS
patients and 30 apparently healthy individuals were used in this
study. Blood samples were obtained for DNA analysis and hormonal
measurements. Genotyping of the TCF7L2 gene was carried out by
using PCR-RFLP method, then the positive samples were subjected
to sequencing analysis. Significant differences were observed in
allele and genotype frequencies of TCF7L2(rs7903146)
polymorphisms among study groups. There was significantly
elevation of T allele in diabetic PCOS Compared with control (19%
versus 7%) and increase risk (OR 1.325%, χ² 4.793). Both CT and TT genotypes were
significantly increased in patients compared with control and increased risk (OR 1.188, χ²
4.829 ; OR 1.424, χ²4.922, respectively ) and PCOS risk with CT genotype (OR 1.035 ,
χ² 7.081). An association was found among CT, TT genotypes and hormonal, biochemical
traits in patients. An association was found among Two genotypes (CT and TT) with the
occurrence of both diabetic PCOS and PCOS in the Iraqi patients women.
Keywords: TCF7L2, PCOS, RFLP, Diabetes mellitus Type2.
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