Abstract

IMPACT OF THE TRANSCRIPTION FACTOR 7-LIKE2 (TCF7L2) GENE POLYMORPHISMS ON THE RISK OF POLYCYSTIC OVARY SYNDROME IN IRAQI PATIENTS

Mohammed T. Al-Tamimi, Ismail.A.Abdul-Hassan*, Saad.S.Al-dujaily and Bushra J.M.Al-musawi

Abstract

The relationship of TCF7L2 gene polymorphisms (rs7903146) with the incidence of PCOS and diabetic PCOS in Iraqi patients (women) was evaluated in this study. Sixty PCOS and diabetic PCOS patients and 30 apparently healthy individuals were used in this study. Blood samples were obtained for DNA analysis and hormonal measurements. Genotyping of the TCF7L2 gene was carried out by using PCR-RFLP method, then the positive samples were subjected to sequencing analysis. Significant differences were observed in allele and genotype frequencies of TCF7L2(rs7903146) polymorphisms among study groups. There was significantly elevation of T allele in diabetic PCOS Compared with control (19% versus 7%) and increase risk (OR 1.325%, χ² 4.793). Both CT and TT genotypes were significantly increased in patients compared with control and increased risk (OR 1.188, χ² 4.829 ; OR 1.424, χ²4.922, respectively ) and PCOS risk with CT genotype (OR 1.035 , χ² 7.081). An association was found among CT, TT genotypes and hormonal, biochemical traits in patients. An association was found among Two genotypes (CT and TT) with the occurrence of both diabetic PCOS and PCOS in the Iraqi patients women.

Keywords: TCF7L2, PCOS, RFLP, Diabetes mellitus Type2.