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Abstract

A COMPARATIVE INVITRO STUDIES OF PHYTOCHEMICAL ANALYSIS AND ANTIMICROBIAL ACTIVITY OF METHANOLIC EXTRACTS OF TAGETES ERECTA AND TAGETES PATULA.

T. Venkata Sowjanya, Mallela Malathi*, Gurugula Mohana Lakshmi Gayathri, Kalakonda Harsha Priya

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Abstract

The focus of the current work is on analysing Tagetes erecta and Tagetes patula for their antimicrobial and phytochemical properties. Humans have employed natural plant products for a variety of uses throughout history. Ayurveda, an Indian system of medicine, primarily employs plant-based treatments or formulations to treat a variety of ailments, including cancer. The market for natural medicines has significant room for growth. Dried flower petals of T.erecta and T. patula were extracted with methanol, macerated for 24 hours at room temperature. The filtered methanolic extracts were evaporated to form thick residue used for Phytochemical screening and evaluating antimicrobial activity seperately .The presence of T.erecta contains Alkaloids, Phenols and Aminoacids. T.patula contains Alkaloids, Phenols and Flavonoids. MHA media was prepared and inoculated with bacterial strains Gram positive bacteria [Bacillus subtilis and Staphylococcus aureus] and Gram negative bacteria [Escherichia coli and Aeromonas sorbia]. For invitro antimicrobial activity by disc diffusion method various concentrations (10 μg/ml, 20 μg/ml, 30 μg/ml, and 40 μg/ml) of methanolic extractions of T erecta and T. patula were prepared in DMSO. The comparative study revealed that of T.erecta exhibited highest zone of inhibition 26 mm for Staphylococcus aureus (gram +ve) and Aeromonas sorbia (gram -ve). flower extracts of T.patula showed less zone of inhibition towards same strains.Results of antimicrobial activities of extracts indicate that T. erecta shows broad spectrum of activity.

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