COMPARATIVE GENETIC ANALYSIS AMONG GENERA CYMBOPOGON AND VETIVERIA BY RAPD, SOUTHERN HYBRIDIZATION AND SEQUENCING
Nirupama Lal* and Sudhir K. Awasthi
Abstract
Two of the most important genera of family ‘Poaceae’ namely
Cymbopogon and Vetiveria were investigated in this study with the
objective to differentiate the species, determining the diversity and
assessing the synteny among them. For this purpose, RAPD, southern
hybridization and sequence analysis were used as tools. Combinely,
after assessing all the parameter, a tentative pattern of evolution for
different accessions of Cymbopogon and Vetiveria zizanioides was
deduced in which all of the species were predicted to be evolved from
a single ancestor through C. confertiflorus (CCo01) or C. nardus (L.)
Rendle var. confertiflorus (CN01) type of genome. All species of
Cymbopogon studied were having the basic chromosome number
X=10. In the synteny study, Vetiveria types of grasses were analyzed to be derived from
wild relatives of Cymbopogon type of genome. Vetiveria was further thought to be of recent
origin in its development compared to Cymbopogon. The presence of similar sequence RAPD
fragments in these two types of genomes were further confirmed through southern
hybridization and sequencing.
Keywords: Cymbopogon; Vetiveria; Genetic diversity; RAPD; Molecular markers; Southern hybridization; Sequencing; Synteny.
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