Abstract

ACUTE AND SUB-ACUTE TOXICITY OF AQUEOUS AND HYDROETHANOLIC EXTRACTS OF THE RECIPE FOR THE LEAVES OF ERIOSEMA ERICI-ROSENII RE FR. AND NEOBOUTONIA MELLERI MÃœLL.ARG. PLAIN

Mambeke H. M., Elion Itou RDG1,*, Boukongo R. P., Etou Ossibi A. W., Morabandza C. J. and Abena A. A.

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Abstract

Eriosima erici and Neoboutonia melleri are two plants used in the form of a recipe in traditional Congolese medicine to treat various pathologies. However, the use of this recipe does not guarantee its harmlessness. It is in this perspective that we estimated the acute and subacute toxicities of the aqueous extracts and the hydroethanolic of the recipe according to the protocols of the OECD n° 425 and 407. The effects of these extracts were evaluated on the general behavior, mortality, weight change as well as haematological and biochemical parameters. Concerning the acute toxicity, the administration of a single dose of 5000 mg/Kg, with the hydroethanolic extract led to a reduction in mobility and polyuria from the first hour following administration with a significant reduction in weight. However, no mortality was observed. The LD50 is estimated to be greater than 5000 mg/Kg. Regarding subacute toxicity, daily administration of a dose of 600 mg/kg of the two extracts for 28 days to rats was well tolerated. However, there are some changes in certain hematopoietic (VGM and white blood cells) and biochemical (AST, ALT, urea, uric acid and tryglycerides) parameters with reversibility of the effects observed with the aqueous extract after stopping treatment.

Keywords: acute toxicity, subacute toxicity, recipe, Eriosema erici and Neoboutonia melleri.