Abstract

CYTOTOXICITY AGAINST TUMOR CELL LINES OF A PURIFIED MIRABILIS ANTIVIRAL PROTEIN ISOLATED FROM ROOT OF MIRABILIS JALAPA

Dipti Kumar Chandraprabha Kale* and Usha Mukundan

Abstract

Background: Root extract of Mirabilis jalapa (Nyctaginaceae) contain an antiviral protein which is type I Ribosomal inactivating protein (RIP), named as Mirabilis antiviral protein (MAP). Objective: The aim of the study was to isolate, purify, identify and study its potential application as an anticancer agent. Methodology: The protein was purified to homogeneity from roots of Mirabilis jalapa by ammonium sulfate precipitation and ion exchange chromatography with CM-Sepharose CL-6B column. The purified protein was identified with MALDI-TOF followed by MASCOT-search. Cytotoxic effect of purified protein against three cancer cell lines, namely MCF- 7, A549, HCT 116 and Vero cells as normal cells by MTT assay was studied. Results: The list of selected ion peaks from the mass spectrum of the purified trypsin digested product was used to compare the result with database. The MASCOT software identified Mirabilis Antiviral Protein (MAP) as the most likely protein. The in-vitro anti-proliferative activity studies proved that HCT116, MCF7 and A549 cell lines were inhibited significantly with increasing of drug concentration. IC50 of MAP was observed to be 150 μg/ml, 175 μg/ml, 200 μg/ml to HCT116, MCF-7, A549 respectively. Discussion: There may be differential expression of MAP receptor type on the different cells used in the present study, which account for the difference in cytotoxic response against cancer cell lines. The cytotoxicity mechanism of isolated RIP from M. jalapa could be due to apoptosis. Conclusion: The present study showed that the plant M. jalapa contains MAP; Type I RIP. This could be utilized as therapeutic agents against cancer.

Keywords: Mirabilis Antiviral Protein, Ribosome Inactivating Protein.