CYTOTOXICITY AGAINST TUMOR CELL LINES OF A PURIFIED MIRABILIS ANTIVIRAL PROTEIN ISOLATED FROM ROOT OF MIRABILIS JALAPA
Dipti Kumar Chandraprabha Kale* and Usha Mukundan
Abstract
Background: Root extract of Mirabilis jalapa (Nyctaginaceae)
contain an antiviral protein which is type I Ribosomal inactivating
protein (RIP), named as Mirabilis antiviral protein (MAP). Objective:
The aim of the study was to isolate, purify, identify and study its
potential application as an anticancer agent. Methodology: The protein
was purified to homogeneity from roots of Mirabilis jalapa by
ammonium sulfate precipitation and ion exchange chromatography
with CM-Sepharose CL-6B column. The purified protein was
identified with MALDI-TOF followed by MASCOT-search. Cytotoxic
effect of purified protein against three cancer cell lines, namely MCF-
7, A549, HCT 116 and Vero cells as normal cells by MTT assay was
studied. Results: The list of selected ion peaks from the mass spectrum
of the purified trypsin digested product was used to compare the result with database. The
MASCOT software identified Mirabilis Antiviral Protein (MAP) as the most likely protein.
The in-vitro anti-proliferative activity studies proved that HCT116, MCF7 and A549 cell
lines were inhibited significantly with increasing of drug concentration. IC50 of MAP was
observed to be 150 μg/ml, 175 μg/ml, 200 μg/ml to HCT116, MCF-7, A549 respectively.
Discussion: There may be differential expression of MAP receptor type on the different cells
used in the present study, which account for the difference in cytotoxic response against
cancer cell lines. The cytotoxicity mechanism of isolated RIP from M. jalapa could be due to
apoptosis. Conclusion: The present study showed that the plant M. jalapa contains MAP;
Type I RIP. This could be utilized as therapeutic agents against cancer.
Keywords: Mirabilis Antiviral Protein, Ribosome Inactivating Protein.
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