Abstract

WHICH ONE IS THE MOST POTENTIAL AS ANTI-DIARRHEA: BLACK TEA LEAVES (CAMELLIA SINENSIS L.) INFUSION OR RED PIPER BETEL LEAF EXTRACT (PIPER CROCATUM RUIZ & PAV)

Sri Agung Fitri Kusuma* and Dasnni Ramdhani

.

Abstract

Objective: This study was performed to compare two plant products containing cathecin which is known as flavonoid compound for bacterial growth inhibition. Our studied plant were black tea leaf (Camellia sinensis L.) infusion and red piper betel leaf extract (Piper crocatum Ruiz & Pav). Methods: Black tea leaves were prepared for extraction using an infusion process, menawhile the red piper betel leaf using a maceration process. Both extract's secondary metabolites were examined using a standard method. The agar diffusion method was used to assess the extract's antibacterial effects, and the macrodilution method was used to determine the extract's minimum inhibitory concentration (MIC). Whereas, the MIC result after an overnight incubation was sub-cultured onto the surface of Mueller Hinton Agar medium to determine the minimum bactericidal concentration (MBC). Results: The findings demonstrated that the infusion of black tea leaves and red piper betel leaf extract have antibacterial activity against Escherichia coli with the same MIC/MBC ranging from 2.5 to 5.0 %w/v. Conclusion: Since both plant extracts have the same values of inhibitory efficacy, they can both be used as an alternate herbal remedy for diarrhea.

Keywords: Camellia sinensis L., infusion, Piper crocatum Ruiz & Pav, extract, diarrhea, Escherichia coli.