Abstract

EVALUATION OF CYTOTOXIC AND EX-VIVO CARDIOPROTECTIVE INVESTIGATION OF ETHANOLIC EXTRACT OF GLYCOSMIS PENTAPHYLLA LEAVES

Prawej Ansari, Shofiul Azam, Md. Reyad-ul-ferdous1*, Jalish Mahmud, Md. Monirul Islam, Sukdeb Goswami1 and Md. Naimul Islam

Abstract

The present study was designed to investigate the cytotoxic activity of methanol extract of Glycosmis pentaphylla on brine shrimp and thrombolytic activity of methanol extract of Glycosmis pentaphylla on human blood. Ethanolic extract of G. pentaphylla was assessed with the Brine shrimp lethality bioassay was used to evaluate cytotoxicity and ethanolic extract of G. pentaphylla assessed with human blood to evaluate thrombolytic effect. The extract showed remarkable cytotoxic activity, LC50 value of the extract was 2.8315μg/ml compared to vincristin sulphate, a reference drug. It also has evaluated as thrombolytic agent compared to streptokinase. It has significant thrombolytic effect which was about 33.144%. These findings demonstrate that the leaves extract of G. pentaphylla have excellent cytotoxic activity and Glycosmis pentaphylla have excellent thrombolytic effect.

Keywords: Glycosmis pentaphylla, bioassay, cytotoxic, LC50, cardioprotective activity.