Abstract

ANTIMICROBIAL PROPERTY OF ACACIA NILOTICA SSP. TOMENTOSA AGAINST SOME CAUSATIVE AGENTS OF UROGENITAL INFECTIONS.

Abd-Ulgadir K. S.*, El-Rofaei N. A. and El-Kamali H. H.

Abstract

This study was aimed to investigate the antimicrobial potential of methanolic and aqueous extracts of leaves and stem barks of Acacia nilotica ssp. tomentosa against five standard bacterial strains and one species of fungi (viz: Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (ATCC 53657), Proteus vulgaris (ATCC 6380), Staphylococcus aureus (ATCC 25923) and Candida albicans (ATCC 7596). As well as, we examined the susceptibility of 133 clinical isolates belonging to seven bacterial genera and one species of fungi to the same tested extracts. The agar well diffusion method was followed to detect the susceptibility of both standard strains and the clinical isolates to different extracts of the plant. The minimum inhibitory concentration (MIC) of extracts was determined using the agar dilution methods. Methanolic and aqueous extracts of Acacia nilotica ssp. tomentosa exhibited potent antimicrobial activity against standard strains and the clinical isolates of Escherichia coli, Proteus mirabilis Staphylococcus saprophyticus, Pseudomonas aeruginosa, Neisseria gonorrhoeae, Enterococcus spp. and Candida albicans. Minimum inhibitory concentrations of methanolic extracts ranged from 6.25-12.5 mg/ml to all tested isolates.

Keywords: Antimicrobial potential; Acacia nilotica ssp. tomentosa; Bark extract: Clinical isolates; Leaves extract.