Abstract

ASSESSMENT OF ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF PIMPINELLA ANISUM SEED EXTRACTS AGAINST STREPTOCOCCUS SANGUINIS AND FUSOBACTERIUM NUCLEATUM -AN IN-VITRO STUDY

Tushar Udaykumar Nadagadalli*, Suchetha Aghanashini, Sapna Nadiger, Darshan Basavaraj Mundinamane, Apoorva Sokke Mallikarjunappa and Anusha D.

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Abstract

Background: Periodontal diseases are conditions due to multifactorial etiology having various treatment approaches. Phytochemicals in the periodontal therapy has been evolved in the recent years with their diverse benefits. Pimpinella anisum (anise) seed is an essential seed with many medicinal properties. AIM: The aim of this study was to assess the antibacterial, antioxidant activity of Pimpinella anisum methanol seed extract under laboratory condition. Methods: In the present study, the methanolic seed extract of Pimpinella anisum was assessed for its antibacterial effects against Streptococcus sanguinis and Fusobacterium nucleatum using well diffusion technique and MIC (minimum inhibitory concentration) was determined by spectrophotometric method using the dye MTT. The antioxidant effects were assessed by 2, 2-diphenyl-1- picrylhydrazyl radical (DPPH) assay, ABTS 2,2'-azino-bis (3 ethylbenzothiazoline-6-sulfonic acid)) and Ferric reducing antioxidant power (FRAP) assay. This was done in triplicates for each concentration of the extracts. The statistical analysis was done by One-way ANOVA Test followed by Tukey's post hoc test was used to compare the mean ZOI & MIC values between different concentrations of Test extract & Tetracycline group. Kruskal Wallis Test followed by Dunn's post hoc test was used to compare the mean MBC values between different concentrations of Test extract & Tetracycline group. Results: The methanolic extract of Pimpinella anisum seed showed highest mean zone of inhibition of 24.33 at 1000μg/ml, mean MIC and MBC values of 0.4320 and 0.320 respectively at 80μg/ml on Streptococcus sanguinis however no inhibitory effect on F. nucleatum and showed antioxidant activity by DPPH, ABTS and FRAP assay. Conclusion: Pimpinella anisum methanol seed extract had antibacterial effect and antioxidant property only on Streptococcus sanguinis. But further studies are required to evaluate antibacterial and antioxidant activity on various other Periodontopathogenic bacteria and under in-vivo conditions.

Keywords: Pimpinella anisum, Anise seed, Minimum inhibitory concentration, S. sanguinis.