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Abstract

RP -HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF CARVEDILOL AND LISINOPRIL IN SYNTHETIC MIXTURE

Nena Jaiswal*, Mitali Dalwadi, Priyanka Malani and Umesh Upadhyay

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Abstract

It is simple, sensitive and accurate RP-HPLC method was developed for simultaneous estimation for Carvedilol and Lisinopril. A reversed– phase high-performance liquid chromatography method is developed and validated for the determination of both drugs. RP-HPLC method was developed using Cyber-sil C18 (250 x 4.6mm, 5μm) column as a stationary phase and Acetonitrile: Methanol: Water (60:20:20 % V/V/V) as mobile phase. The flow rate was maintained at 1 ml/ min and detection was carried out at 218 nm where Lisinopril (LIS) and Carvedilol (CAR)) have significant absorbance. The retention times of Lisinopril (LIS) and Carvedilol (CAR) was found to be 2.853 minute and 6.480 minute respectively. RP-HPLC method is linear in the concentration range of 510-30 μg / ml for LIS and 20- 60 μg/ ml for CAR, with correlation coefficient found to be 0.9991 for LIS and 0.9993 for CAR. The recovery was in the range of be 99.04% -100.04% for LIS and 99.57% - 100.16% for CAR, respectively. The detection limits for LIS and CAR were found to be 1.38 μg/ml and 0.91 μg/ml respectively, while quantitation limits were found to be 4.17 μg/ml and 2.77 μg/ml respectively. The method was found to be accurate, precise, specific, selective, repeatable, robust and reproducible.

Keywords: Carvedilol (CAR), Lisinopril (LIS), Reversed-Phase-High performance Liquid Chromatography (RP-HPLC).


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