Abstract

SPECTROFLUORIMETRIC METHOD DEVELOPMENT AND VALIDATION OF TETRACYCLINE IN CAPSULE DOSAGE FORM

Sangavi S., Lakshmi R. Mohanan, Kavinila S., Sathriyan M., Hardha B.* and Senthil Kumar M.

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Abstract

A Spectrofluorimetric method has been developed for estimation of Tetracycline in capsule form. It turns out the pH 8 is the one that gave the highest signal then the other acidic range, Tetracycline is more fluorescent under these conditions, i.e., stable at pH > 7. The accuracy recoveries were between 99.88% and 101.10%. For intra-day and interday precision the relative standard deviation (RSD) between 1.57% and 2.88% were lower than 5%. Linearity was established by the least squares regression method of the calibration curve. This standard curve was obtained in the range of 0.25 to 1.5 μg/ml. The correlation coefficient is greater than 0.9998. The results show an excellent correlation between the fluorescent intensity and the concentration of the analyte. The content of our samples is quantitatively determined. The results found vary from 94.5% to 108.5%. Margins of (100 ± 10) % have been set as the acceptable limit for the method used. The results of the samples met the official acceptance criteria.

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