Abstract

ANTIOXIDANT AND REACTIVE OXYGEN SPECIES INDUCTION USING PURIFIED NATURAL LIGNAN DIMMER ISOLATED FROM MYRISTICA FRAGRANS SEED

Essam F. Al-Jumaily*, Ahmed F. Al-Shanon* and Shymaa I. Al- Barzanchi*

Abstract

In this paper the free radical scavenging activities were studied using stable free radical compound 1,1-Diphenyl-2-Picryl-hydrazil (DPPH). Results showed that 100, 10, 1 and 0.1 μg/ml of purified lignan had 76.7 %, 65%, 28% and 8% scavenging activity respectively, while the same concentrations of partial purified lignan had 44.3%, 18.5%, 11% and 0% scavenging activity respectively. Reactive oxygen species (ROS) induction was studded in MCF-7 cells (breast cancer) in the presence of 200, 100 and 50 μg/ml of purified lignan, results reveled that 20, 11.48 and 9.61% reduction in reactive oxygen species was occurred respectively. We can concluded that the natural lignan dimmer had free radical scavenging effect and could reduce the reactive oxygen spices induction, and had a potential therapeutic effect on breast cancer cells.

Keywords: Pure lignin dimmer, Myristica fragrans seed, MCF-7 cell, reactive oxygen species (ROS).