Abstract

BIOLOGICAL CORRELATION BETWEEN TNF-? 238 G/A GENE POLYMORPHISMS AND GALLSTONE FORMATION AMONG IRAQI PATIENTS

Salih Abdul Mahdi Khadim*

Abstract

Gallstone is one of most biliary disorder affected by several risk factors such as Immune factors like inflammatory cytokines as well as ethnic variants which are important in most disease. Several studies estimated some cytokines can be detected in bile fluid. Previous findings demonstrated the correlation between cytokines and gall stone formation provided the unexpected result that cytokines deficiency markedly increased the formation of gallstones as well as reported that several specific cytokines have been implicated in cholesterol and lipoprotein metabolism. So in this research, tumor necrosis factor alpha (TNF-α) gene polymorphisms at position 238 G to A were studied, and its correlation with gallstone formation were estimated. On 85 gallstone Iraqi patients and 86 normal subjects, by amplification refractory mutation system polymerase chain reaction (ARMS –PCR) technique; TNF-α 238 G/A gene polymorphism were evaluated. Present findings showed no significant differences in distribution of TNF-α GG and GA genotypes between patient and normal control group (P < 0.05). Whereas the correlation between AA mutant genotypes and gallstone formation was significant statistically (P > 0.05) and (OR= 0.38,95 % CI = 0.12 – 1.11).These results suggested that AA mutant homogenous genotypes played a protective role against gallstone formation among Iraqi patients.

Keywords: TNF-? 238, polymorphism, gallstone, Iraqi, patients.