Abstract

FORMULATION AND CHARACTERIZATION OF NOVEL THERMORESPONSIVE AND BIOADHESIVE IN-SITU GEL CONTAINING MUPIROCIN FOR ANTIBACTERIAL DELIVERY

Dr. A. Abdul Hasan Sathali* and B. Keerthana

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Abstract

The present research work was aimed to prepare and evaluate Thermoresponsive and Bioadhesive In-situ gel containing mupirocin for antibacterial delivery, By using the in-situ gelling system reducing the dosing frequency of administration and improve the patient compliance. The temperature sensitive in-situ gel were successfully developed by the cold method of preparation by using poloxamer 407, poloxamer 188 and carbopol 934p used as a Thermoresponsive and Bioadhesive polymers. The Box behnken design was employed to investigate the impacts of the polymer compositions used as a independent variables on the gelation temperature (Tsol-gel), solution state viscosity and spreadability as a dependent variables. The FT-IR studies confirmed there is no interaction between the polymers and drug. The optimized formulation have gelation temperature Tsol-gel (35ºC), pH(5.57±0.2), spreadability(5.7cm±0.5) Solution state and gelling state viscosity (86cps) and (3649 cps). Drug content (98.76%), the bioadhesiveness of optimized formulation was (2853±0.75) this leads to prolonged residence time of formulation with skin. The in-vitro diffusion studies showed the optimized mupirocin in-situ gel sustained the drug release (72.32%) from the polymeric micelles over the period of 12 hrs than market formulation, and expected to release the medicament some extend period. The In-vitro antibacterial studies of optimized formulation sensitive against staphylococcus aureus. This study showed the optimized mupirocin In-situ gel formulations would be a better alternative to conventional formulations to deliver the medicament in the treatment of primary and secondary bacterial skin infections and also improve the patient compliance by minimizing the drawbacks associated with commercial products.

Keywords: Mupirocin, in-situ gel, Thermoresponsive, Box behnken design, Bioadhesive Poloxamer 407.