Abstract

DEVELOPMENT OF STANDARIZATION PARAMETERS FOR HYOCYAMUS NIGER L SEEDS WITH SPECIAL REFERENCE TO ITS PHARMACOGNOSTICAL, PHYSICO-CHEMICAL AND HPTLC FINGERPRINTING STUDIES

J. John Christopher*, S. A. Wasim Akram, S. Mageswari, Mary Shamya Arokia Rajan, S. Tirumala Santhosh Kumar, N. Zaheer Ahmed and Rampratap Meena

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Abstract

Hyacyomus niger (Linn.), commonly known as ―Black henbane,‖ is well known for its therapeutic actions as Central Nervous System depressant and anti-cholinergic agent. The presence of active tropane alkaloids like atropine and scopolamine in the seeds makes them pharmacologically effective. Therefore, in this present study, the Highperformance thin layer chromatography (HPTLC) analysis and Pharmacognostical characters of seeds of Hyacyomus niger L. was evaluated in order to establish the scientific data for the future studies and reference. The pharmacognostic studies show some of the key character consists of single layer of palisade like very thick walled sclerenchyma cells followed by few layers thin walled crushed parenchyma cells on the inner side. The HPTLC fingerprinting and densitometric analysis of ethanol extract of Hyacyomus niger L(Seed) was carried out using CAMAG HPTLC system. The phytochemical profile of the plant was presented in the tables showing the total number of peaks, peak heights, peak area, percent area, and Rf values. The study concluded that H. Niger seeds of ethanol extract contains a rich variety of Phytochemicals with 14 spots corresponding to 14 different phytoactive compound and its Pharmacognostical features evaluated serves as the evidence in finding the adulterants and thus helps in the correct identification of the plant material providing standard quality control reference for the new research perspective.

Keywords: Hyacyomus niger L, HPTLC, Ethanol extract, Pharmacognosy, Densitometric Chromatogram.