Abstract

DEVELOPMENT AND VALIDATION OF UV-SPECTROSCOPIC METHOD FOR THE ESTIMATION OF OXETACAINE IN BULK AND PHARMACEUTICAL DOSAGE FORM

Srimyvizhiy G. M.*, K. B. Ilango, Ajeeth kumar S., Boomika N. and Ponnar Shankar A.

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Abstract

A new, trouble-free and sensitive Spectrophotometric method in ultraviolet region has been developed for the estimation of oxetacaine in bulk and pharmaceutical dosage form. It was the main objective of this study for know about the oxetacaine should be still any research there is no developmental method in UV – spectroscopy. It is the first article to reveal the method development in spectroscopy for oxetacaine. Quantitation was based on peak area at 206 nm. The method obeys the Beer’s law in the concentration range from 1.5 – 5.5 μg/ml. The linearity was observed in the above concentration. Here we using only methanol as a solvent to reduce our cost for double solvent using in estimation. The limit of detection for oxetacaine was obtained in satisfactory limits such as 0.051μg/ml. The limit of quantification of oxetacaine was obtained in reasonable limits such as 0.156μg/ml. The percent recovery of oxetacaine was found to be 99. 56%. The percentage relative standard deviation for the intraday and interday precision was obtained in the range of 1.191 & 1.349%. The obtained results were confirmed that the proposed method is specific, rapid, accurate, reproducible and suitable for the routine determination of oxetacaine. And also the above proposed method was validated according to the ICH guidelines.

Keywords: Oxetacaine, UV – spectroscopic calibration curve method, Methanol, validation.