Abstract

FORMULATION DEVELOPMENT AND IN-VITRO EVALUATION OF TETRAZOSIN LOADED CHITOSAN NANOPARTICLES FOR THE TREATMENT OF HYPERTENSION

Daggumati Pravallika, *V. Jhansi Priya Marabathuni and Naidu Narapusetty

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Abstract

The purpose of this research was to prepare Tetrazosin loaded Chitosan Nanoparticles for controlled release of drug, to improve the solubility, reduce the dosing frequency, thereby increasing patient compliance to the therapy. Tetrazosin is formulated as Nanoparticles by ionicgelation method using Chitosan as polymer, Sodium tripolyphosphate as a polyanionic agent (cross linking agent) and the lyophilized nanoparticles filled in hard gelatin capsules. The particle size analysis was done by Horriba scientific Nano SZ 100 particle size analyzer showed that mean particle size 188.3 nm and Z- Average 229.0 nm respectively. The Zeta potential study was done by Horriba scientific Nano SZ 100. The Zeta potential for the optimized formulations F5 was found to be 25.8mV and shows that the formulation is stable. Post formulation parameters (uniformity of weight, disintegration test, drug content, and in vitro drug release) for nano particulate capsules were evaluated. The results were found to be complying with official specifications. The dissolution data of the optimized formulation was fitted to various kinetic models and the formulation F5 was best fitted to Zero order kinetics. The slope of the Korsmeyer Peppas plot indicating the diffusion was Anomalous diffusion (Non-Fickian diffusion). From the overall results, it is clear that the formulations F5 containing 0.3% polymer concentration (Chitosan) is the optimal formulation, as it produces controlled drug release.

Keywords: Chitosan Nanoparticles, particle size Zeta potential, kinetic study, Non- Fickian diffusion.