Abstract

IN – VITRO FUNGITOXIC EVALUATION AND GCMS ANALYSIS OF CALOTROPIS PROCERA

Deepika Srivastava* and Padma Singh

Abstract

The present investigation was carried out to find the fungitoxic potential of different extracts of Calotropis procera, and GCMS analysis of the best extract. Four different extracts of Calotropis procera (leaves) were prepared using four different solvents viz aqueous, methanol, acetone and ethanol. These extracts were analyzed for antifungal activity against different isolates of Alternaria alternata. Ethanolic extract completely inhibited the growth while methanol inhibited 76% - 81%, acetone 86% - 91% and aqueous gave least inhibition i.e. less than 3%. As ethanol showed best result. MIC and MFC was carried out for ethanolic extract and MIC for all isolated was noted to be 6.25 mg/ml and MFC for all isolates was 12.5 mg/ ml. Phytochemical analysis was also carried out which showed the presence of different secondary metabolites in different extracts. GCMS of ethanolic extract of Calotropis procera was also carried out which revealed the presence of Phytol (9.77%), n-Hexadecanoic acid (7.71%), Methyl commate A (7.71%), Lup - 20 (29) - EN - 3- YL acetate (5.73), as major compounds. The present study provides the insight that Calotropis procera ethanolic extract can serve as biofungicide for controlling disease caused by Alternaria alternata. Thus there will be natural origin of the fungicide which will have no side effects.

Keywords: Calotropis procera, Alternaria alternata, MIC, MFC, GCMS.