Abstract

A STUDY ON ANTIOXIDANT AND GASTRO-PROTECTIVE EFFECTS OF TRACHYSPERMUM AMMI LINN EXTRACT USING DIFFERENT IN-VITRO TECHNIQUES

Priyanka Pandey*, Purva Jain and Wasim Raja

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Abstract

Herbal medicine also called botanical medicine or phytomedicine refers to using a plant's seeds, berries, roots, leaves, bark, or flowers for medicinal purposes. Herbalism has a long tradition of use outside of conventional medicine. It is becoming more main stream as improvements in analysis and quality control along with advances in clinical research show the value of herbal medicine in the treating and preventing disease. The use of local plants in folk medical practices has a long history. The resource base of the traditional medical practices prevalent in rural and tribal villages of India and abroad is mainly the plants. Trachyspermum ammi which is commonly known as Ajowan. Among traditional potential herbs used as spice in day to day life, ajwain (Trachyspermum ammi L.) belonging to family Apiaceae. The phenolic compound in fruits, vegetables, herbs and spices possess potent antioxidant, anti-inflammatory, antigenotoxic and anticancer activities. The dried seeds of Trachyspermum ammi were extracted with methanol using a Soxhlet extractor. The total phenolics content of seed as determined by Fenton reaction and was found to be good antioxidant activity as different dose concentrations. The antioxidant activity of seed extract was carried out with ascorbic acid as a standard reducing agent. The present results were made with the use of UV-Visible Spectrophotometer. In this plant Trachyspermum ammi Extract there was a remarkable concentration dependent free radical scavenging and reducing power was exhibited. We were also found the gastroprotective activity of T. ammi extract as compared to Liv52 standard drug and control liver enzymes was also significant using SGOT and SGPT enzymes. T. ammi extract treated histology slide of the liver a classic liver lobule the hepatocytes are polyhedral with eosinophilic cytoplasm and a usually central nucleus. Between the cords of hepatocytes are endothelially lined sinusoids showing normal histology of liver as compared to control and Liv52 treated groups and significant reduction were also found to intestine histology as compared to untreated control. In conclusion the present study indicates that Trachyspermum ammi extract may be a potential source of natural antioxidant and gastroprotective effect. Trachyspermum ammi appears as an excellent option to enrich the diet with antioxidant phenolic compounds as well as gastroprotective agent.

Keywords: Gastro-protective, Antioxidant activity, Fenton Reaction, Hydroxyl radical, Trachyspermum ammi, SGOT and SGPT enzymes.