Abstract

SYNTHESIS, CHARECTERIZATION AND EVALUATION OF INVITRO ANTI-TUBERCULAR ACTIVITY OF NOVEL SUBSTITUTED TRIAZOLE DERIVATIVES

*Dr. Channamma G. M., Namrata A. T., Dr. Kishore Singh C. and Dr. Ashok Kumar M.

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Abstract

Ethyl Palmitate (i) was prepared by the reaction of Palmitic acid with absolute ethanol in the presence of concentrated sulphuric acid by refluxing. Ethanolic solution of Ethyl Palmitate (i) treated with Hydrazine hydrate and refluxed to yield Palmitohydrazide (ii). 5- pentadecyl-1,3,4-oxadiazole-2(3H)-thione (iii) was prepared by treating Palmitohydrazide (ii) with solution of Potassium hydroxide then Carbon disulfide was added, refluxed, cooled, Hydrochloric acid was added to neutralise, the solid filtered and recrystallized. 4-amino- 5-pentadecyl-4H-1,2,4-triazole-3-thiol (iv) was synthesized by an Ethanolic solution of 5-pentadecyl-1,3,4-oxadiazole-2(3H)-thione (iii) and Hydrazine hydrate heated for 3hrs and poured in ice, filtered and recrystallized. 4-amino-5-pentadecyl-4H-1,2,4-triazole-3-thiol (iv) upon treatment with different substituted aldehydes in Ethanol followed by addition of few drops of Sulphuric acid was refluxed for an appropriate time, resulted in the formation of above titled compounds [NT-(a-d)]. All the synthesized compounds were characterized on the bases of its TLC, physical constant, spectral studies. Further these compounds were evaluated for their anti-microbial and anti-tubercular activities.

Keywords: Anti-tubercular, Substituted Triazole, Microplate Alamar Blue Assay, Antimicrobial.