Abstract

DETERMINATION OF BIOACTIVE COMPOUNDS FROM THE PETROLEUM ETHER LEAF EXTRACT OF MORINGA OLEIFERA AND PHYLLANTHUS EMBLICA USING GC-MS ANALYSIS

Malliga Elangovan*, M.S. Dhanarajan, I. Elangovan

Abstract

Objective: The aim of the present study was to determine the presence of number of biologically active phytoconstituents in the petroleum ether leaf extracts of Phyllanthus emblica and Moringa oleifera using Gas Chromatography- Mass Spectrometry (GC-MS). Methods: In the present investigation, petroleum ether extracts of Phyllanthus emblica and Moringa oleifera leaves were screened for the presence of potent bioactive phytocompounds using FT-IR and GC-MS analysis which could be a possible source of extracting the therapeutically useful products. Results: FT-IR analysis of both the plant extracts exhibit the presence of different functional groups ranging from O-H Alcohol, CH Alkanes, Esters C=O stretch, C-N amine, Ketone, C=O as their key functional groups. The major bioactive components present in the petroleum ether leaf extract of Moringa (PELMO) screened by GC-MS analysis revealed the presence of eight bioactive compounds specifically Unsaturated alcohols, Saturated hydrocarbons, Unsaturated Fatty acid, Alkane hydrocarbon, Vitamin E, Epoxide and Triterpenoids whereas Phyllanthus emblica (PELPE) demonstrated the presence of seventeen bioactive compounds which includes Alcoholic compound, Saturated hydrocarbons, Unsaturated Fatty acid, Fatty Alcohol, Alkane hydrocarbons, Vitamin E, Ester compounds, Plant Sterols and Triterpenes. Conclusion: Based on the results obtained in this study, it could be concluded that the crude extract of M. oleifera and P.emblica leaf powder contains various bioactive chemical compounds of medicinal properties which further justify the application of these traditional plants in the discovery of novel therapeutics.

Keywords: GC-MS, FT-IR, Petroleum ether extract, Phyllanthus emblica, Moringa oleifera.