Abstract

AN EXPERIMENTAL STUDY TO EVALUATE THE EFFECT OF KRISHNA MUSALI CHOORNA AND KRISHNA MUSALI KSHARA IN CERVICAL CANCER CELL LINES

Nayana N.*, Suchetha Kumari and Vishwanatha

.

Abstract

Cervical cancer known to be the most common cancer among women. Human Papilloma Virus (HPV) is considered as the main causative factor of cervical carcinoma. HPV infection is mainly transmitted through sexual contact which results in to Cervical Intraepithelial Neoplasia. Early detection and regular cervical screening will help to prevent the progression of pre-cancerous lesion in to malignancy. Based on the clinical features of CIN and cervical carcinoma we can corelate to Arbuda, Granthi or other gynaecological diseases like Tridoshaja Yonivyapat, Kunapagandhi Artavadusti, Tridoshaja Asrugdhara etc in Ayurveda Classics. Krishna Musali is one among Dasapushpa, the ten group of drugs having anticancer properties. Krishna Musali is mentioned in Granthi Chikitsa, Kshara nirmana vidhi etc. Kshara can destroy the precancerous lesion or malignant cells along with elimination of morbid dosha from the site of lesion by its corrosive nature. Methodology: HUMAN EMBRYONIC KIDNEY 293, SiHa and HeLa cell lines were obtained from NCCS, Pune. Cytotoxic effect of Krishna Musali Choorna and Krishna Musali Kshara in HEK293 and Anticancer activity in SiHa and HeLa cervical cancer cell lines were done by assessing cell viability by MTT Assay at SDM Research centre, Udupi. Result: Krishna Musali Kshara showed more cytotoxicity than Krishna Musali Choorna. In SiHa cell line Krishna Musali Choorna and Kshara showed cell viability of 33.83% and 3.64 % respectively. In HeLa cell line Krishna Musali Choorna and Kshara showed cell viability of 16.21% and 6.82 % respectively. Conclusion: Krishna Musali Choorna and Krishna Musali Kshara showed moderate cytotoxicity against HEK293. Krishna Musali choorna showed more anticancer activity in HeLa compared to SiHa cell line. The Krishna Musali Kshara showed more anticancer activity in SiHa compared to HeLa cell line.

Keywords: Carcinoma cervix, CIN, Cell lines, Krishna Musali Choorna and Kshara, MTT Assay.