Abstract

ESTIMATION AND VALIDATION OF QUINIODOCHLOR BY HYDROTROPIC SOLUBILIZATION METHOD

Ajith Babu T. K.* and Pradija Sasidharan

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Abstract

Various organic solvents such as methanol, chloroform, dimethylformamide and acetonitrile have been employed for solubilization of poorly water-soluble drugs to carryout spectroscopic analysis. Drawbacks of organic solvents include their higher cost, toxicity and pollution. Hydrotropic solubilization may be a proper choice to preclude the use of organic solvents. It involves the addition of large amount of second solute to increase the aqueous solubility of first solute. These hydrotropes are economic and pollution-free. In the present investigation, 40% urea solution was employed as hydrotropic solubilizing agent to solubilize poorly water-soluble drug Quiniodochlor. Quiniodochlor exhibits absorption maximum at 267nm. In this method, no interference from any additives and diluents in the estimation of drug was seen. Beer’s law was found to be obeyed in concentration range of 5-25μg/ml. The results of analysis have been validated as per ICH guidelines. The method is accurate, precise and economical.

Keywords: Hydrotropic solubilization, Quinidiochlor, Uv spectroscopy.