Abstract

ISOLATION, CHARACTERIZATION AND MOLECULAR IDENTIFICATION OF BURKHOLDERIA CEPACIA FROM CURCUMA LONGA USING 16S RRNA SEQUENCING

Prasanthi Donipati* and Dr.S.Harasreeramulu

Abstract

Curcuma longa is well known for its use as spice and medicine. The remarkable feature of the plant is the presence of rhizome, which provides an interesting habitat for association by various groups of bacteria. The present study deals with isolation, identification and analysis of bacteria from Curcuma longa through 16S rRNA based molecular technique and samples were obtained from Curcuma longa. The Bacterial strain was isolated and characterized using various biochemical tests and confirmed through molecular approach and 16S rRNA gene was amplified using suitable primers. The amplified 16S rRNA gene sequence was compared with the sequence in NCBI sequence database the bacterial strain was identified. Phylogenetic and molecular evolutionary analyses were conducted using 16S rRNA sequencing. The sequences when submitted to NCBI gene bank database using BLAST showed 99 – 100% maximum identity and have shown good similarity with Burkholderi sps. 2xiao7 strain Burkholderi cepacia strain RRE5, Burkholderi cepacia strain RRE3, Burkholderi cepacia strain CG4, Burkholderi cepacia T-34gene. In the present study we investigated extracts of Curcuma longa rhizomes. Among the isolates, Burkholderi cepacia isolate was the most active.

Keywords: Curcuma longa, rhizomes, Burkholderia cepacia, NCBI.