Abstract

DETECTION AND ESTIMATION OF FLAVONOIDS AND PHENOLIC ACID IN INULA RACEMOSA AND SAUSSUREA COSTUS EXTRACTS BY HPTLC TECHNIQUE

Hemachandran V., Kabilan T., Dibesh S., Dhanus CTR, Jahan R. and Arivukkarasu R.*

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Abstract

The main purpose of the study is to observe the flavonoids and phenolic acids in two herbal raw materials inula racemosa and saussurea costus from Asteraceae family respectively. Traditional siddha practitioners instead of inula racemosa using saussurea costus as substitute in herbal formulation. Our aim of interest to detect and estimate antioxidant markers in inula racemosa and saussurea costus. Results of the study clearly revealed that inula racemosa exhibits quercetin, rutin and gallic acid and saussurea costus exhibit quercetin, gallic acid, caffeic acid. Quercetin was found to be 0.383%, 0.122% in inula racemosa, and 0.042% and 0.1301% in Saussurea costus and rutin was found in 0.007% in inula racemosa and gallic acid was found in 0.02 in inula racemosa and 1.36% and 0.0323% in saussurea costus and caffeic acid was found to be 0.2096% and 0.5811%. In conclusion quercetin and gallic acid was present in both plant; rutin was present only in inula racemosa, and caffeic acid was present only in saussurea costus and vitixen was not present in both plant. Quercetin content is more in inula racemosa when compare to saussurea costus, But Gallic acid is comparatively more in saussurea costus than inula racemosa.

Keywords: Caffeic acid, Gallic acid, HPTLC, Quercetin, Rutin.