Abstract

CARDIOPROTECTIVE ACTIVITY OF COMBINED METHANOL LEAVES EXTRACTS OF PHYLLANTHUS AMARUS AND TRICHOSANTHES CUCUMERINA ON ISOPROTERENOL INDUCED MYOCARDIAL INFARCTED WISTAR ALBINO RATS

*Achilike J. J., Joel O. M. and Amaralam E. C.

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Abstract

This present study was aimed at evaluating the cardio protective activity of combined methanol leaves extracts of Phyllanthus amarus and Trichosanthes cucumerina on isoproterenol induced myocardial infarcted wistar albino rats. Healthy 48 wistar albino rats of both sexes between 8-12 weeks of age weighing 150 -200g was used for this study. The rats were divided into 8 groups, each consisted of 6 animals. Group 1 and 2 served as the negative control and positive control while group 3 to 5 received 500:500 mg/kg, 750:250 mg/kg, 250:750 mg/kg b.w. of Phyllanthus amarus and Trichosanthes cucumerina respectively, while group 6 and 7 where treated with 1000 mg/kg of Phyllanthus amarus and 1000 mg/kg of Trichosanthes cucumerina. Group 8 received standard drug (verapamil). The treatment lasted for 30 days. At the end of the treatment, blood was collected by puncturing the retro-orbital plexus and serum was collected. Serum creatinine kinase-MB, Troponin-1, MDA, SOD and catalase were determined using standard laboratory methods. Results showed that there was significant (p

Keywords: Cardio protective, myocardial infarction, Phyllantus amarus, Trichosanthes cucumerina.